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KMID : 0043319960190050381
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Archives of Pharmacal Research
1996 Volume.19 No. 5 p.381 ~ p.385
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Different Distribution of the α2 Na+, K+-ATPase Isoform between Rat Atria and Ventricles
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Lee Jeung-Soo
Lee Shin-Woong
Wallick Earl-T
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Abstract
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Rat ventricles respond with a biphasic positive inotropic effect to ouabain, low-dose and high-dose effects but rat atria with only a monophasic high dose effect. In an effect to understand the difference in response to ouabain of two tissues between rat atria and ventricles the levels of the -isoform of the , -ATPase which has higher affinity for ouabain than the -iso-form were determined by a ouabain binding assay. The yield of protein per gram wet weight was about 47 mg for atria and 100 mg for ventricles. The values of ouabain for the high-affinity ouabain binding site were nearly the same (230 nM) in the atria and ventricles. However, the numbers of the -isoform per mg protein were approximately half in the atria. When the binding data were expressed in unit per gram tissue wet weight, the numbers of -isoform in the atria was about 25% of that in the ventricles. THese results demonstrate that the -isoform of the -ATPase in the rat atria could be detected by ouabain binding assay and the levels of this isoform are too low to show the low-dose effect of ouabain.
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